FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7155180
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG137500.089.366785
CGTATAC60950.088.996885
GTATAGG146600.088.93876
GTATACC58650.088.680586
GTATACG72550.088.646926
TATACGG84000.088.5512857
CGTATAG88800.088.150885
GGTATAC83150.087.971275
CCTATAG53800.087.5813755
GTATAGC77550.087.218896
CTATACG61150.087.0746156
GCTATAC75750.086.657835
TATAGGC92400.086.001577
AGTATAC62000.085.651025
TTATACG92500.085.18976
CTATAGG92750.085.164926
GCGCCTA5200.085.003782
TATACTG120400.084.661447
GTTATAG154450.084.356845
TATAGCC59350.084.193287
Produced by FastQC (version 0.11.5)