FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light081.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light081.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8505438
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG85900.100994210997717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG167900.089.878466
GGTATAG150550.089.635935
GTATACG77950.089.271056
CGTATAG98350.088.86595
CGTATAC70750.088.688655
CCTATAG64450.088.587665
TATACGG94250.087.79227
GTATAGC94600.087.668236
GTATACC72600.087.344146
GCTATAC84700.086.9801255
GGTATAC93300.086.497475
CTATACG71200.086.459786
AGTATAC79250.085.649475
TATAGGC110150.084.951467
TATAGCC73250.084.818247
GATATAG129000.084.6895455
CCTATAC65000.084.476555
TTATACG105050.084.418456
GCTATAG112400.084.223385
CTATAGG111300.083.988366
Produced by FastQC (version 0.11.5)