FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6457716
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG116100.090.375865
GTATAGG117550.089.50356
GCTATAC61650.088.604075
CGTATAG67300.088.576325
CCTATAG44950.088.130315
CGTATAC50950.088.00695
TATACGG69000.087.839647
GTATAGC67500.087.399066
GTATACG62450.086.556326
TATAGCG71750.086.19427
TATAGGC74850.086.051187
CTATACG52750.085.90436
TATAGCC48650.085.528377
GTATACC49750.085.0694356
CCTATAC48650.084.4543845
TTATACG75800.084.408726
TATACTG103300.084.009267
CTATAGG75550.083.556346
GGTATAC72050.083.52795
GCGCCTA3700.083.5025942
Produced by FastQC (version 0.11.5)