FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light079.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light079.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4206544
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG78750.090.356525
GTATAGG83250.089.352326
TATACGG49000.088.6037
CTATACG36250.088.580276
CGTATAC34450.088.382675
CGTATAG48050.088.2787865
GTATACG41650.088.158216
GTATAGC48150.086.615686
TATAGCC35100.086.3399667
GCTATAC44200.085.758855
CCTATAG30900.085.62395
TATAGCG53000.085.590547
AGTATAC37450.085.36145
ATACGGC10800.084.882148
CCGTATA37950.084.4870764
TATAGGC52150.084.4352347
GTATACC36200.084.372456
CCTATAC32100.084.34675
GGTATAC47950.084.005075
TTATACG52050.083.411086
Produced by FastQC (version 0.11.5)