FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light078.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light078.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4141751
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG76600.090.718586
GGTATAG74350.089.503055
CCTATAG30700.088.653725
TATACGG45450.088.308717
CGTATAG47500.088.297375
CGTATAC33200.088.129915
GCTATAC42750.088.108495
GTATACC34250.087.647036
CTATACG35400.086.6782156
GTATACG37550.086.5220646
TATAGCG50200.086.103017
TATAGGC53850.086.088437
GTATAGC45650.086.048926
AGTATAC34700.085.825975
TTATACG49350.085.372336
GGTATAC44450.084.952485
CTATAGG51950.084.939726
TATAGCC34250.083.902627
ATAGGGC16200.083.854528
GTTATAG91150.083.793455
Produced by FastQC (version 0.11.5)