FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light077.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light077.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4396609
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG83900.089.336385
TATACGG52950.088.98757
GTATAGG82100.088.9808356
CGTATAC40800.088.943985
GCTATAC44400.088.365025
CTATACG36200.088.1747066
GTATACG45500.088.10786
CCTATAG34000.087.872925
CGTATAG48950.087.234915
GTATACC40550.086.915326
GTATAGC49350.085.757846
CCTATAC33300.085.72625
GGTATAC49450.085.296265
TATAGCG54750.085.1075747
AGTATAC41550.085.052145
CTATAGG54100.084.637556
TATAGGC52200.084.442447
TTATACG54800.084.249836
CCGTATA38000.083.998014
ATACGGC10650.083.847788
Produced by FastQC (version 0.11.5)