FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4016532
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC34800.089.9480745
CGTATAG48500.089.807645
GCTATAC41350.089.254725
TATACGG41250.088.7802057
GTATACG37000.088.707846
GTATACC33000.088.3771746
GTATAGG79200.088.3411946
GGTATAG71950.088.06655
GTATAGC45800.087.738586
CTATACG30550.087.6907046
CCTATAG31700.086.7570955
TATAGGC47900.086.5694057
TATAGCC33150.084.681727
GGTATAC45800.084.627285
CCTATAC30050.084.565855
TATAGCG51350.084.4531867
GCGTATA57300.084.304754
TTATACG48150.084.1469656
TATACTG65150.083.989267
CTATAGG52050.083.95626
Produced by FastQC (version 0.11.5)