FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light075.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light075.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3291526
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG33240.10098659406001959No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG59750.091.0258565
GTATAGG63400.090.5054866
CGTATAG37150.089.630625
GCTATAC32500.088.4238055
TATAGCG40700.087.9982767
TATACGG37700.087.819037
CGTATAC24000.087.084065
GTATACG28900.086.782736
GTATAGC35800.086.774476
CTATACG27800.086.286696
CCTATAG25000.085.3107155
GTATACC27050.085.167076
GCTATAG43950.085.057595
CTATAGG44100.084.768286
ATACGCG6400.084.6075068
TATAGGC41200.084.393917
ATACGGC7600.084.373148
CGCTATA32800.084.284244
TATAGCC26750.084.168927
TTATACG43350.083.93386
Produced by FastQC (version 0.11.5)