FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light074.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light074.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4303665
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG63250.1469677588752842No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT46920.1090233556747563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG79450.090.815185
GTATAGG89050.089.879486
CGTATAC33950.089.4037255
TATAGCG62900.089.185417
TATACGG48150.089.081197
CCTATAG31250.088.616195
CGTATAG47800.088.14355
GTATAGC47950.088.065886
GTATACG43750.086.9659046
CTATACG36000.086.9515846
CTATAGG56850.086.477766
GGTATAC45700.086.477225
GCTATAC42950.086.373885
TATAGGC52500.085.862097
TATACTG69950.085.49347
GTATACC34050.085.0956346
AGTATAC38800.084.961535
TTATACG55350.084.8737266
GTTATAG98650.084.792385
TATAAGG70400.084.744517
Produced by FastQC (version 0.11.5)