FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6503665
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG112900.090.875865
GTATAGG119950.089.771816
CGTATAG70000.089.2989965
CGTATAC54200.089.127225
TATACGG72800.088.08287
GTATACG62300.087.832086
GCTATAC69650.087.5654145
CTATAGG72750.087.555726
CTATACG52600.087.413476
CCTATAC44800.087.259075
GTATAGC68100.087.256736
CCTATAG47600.087.015835
GTATACC50950.086.981356
GGTATAC71750.085.863155
GCTATAG84100.085.397375
GTTATAG146350.085.034925
GCGTATA91200.084.321974
TATAGGC72650.084.211037
TATAGGT144800.084.0097667
TATACTG108750.083.249637
Produced by FastQC (version 0.11.5)