FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light072.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light072.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4983346
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG88800.089.59416
GGTATAG88300.089.564415
CGTATAC41750.089.19525
CGTATAG56450.088.8555
CTATACG40650.088.8036046
GTATACG48100.088.676566
TATACGG55000.088.5194557
GTATAGC55050.087.74886
GCTATAC52850.086.9986045
TATAGGC56500.086.4215857
CCTATAG39550.086.3503955
GGTATAC54300.086.1625065
TATAGCG67650.085.6583257
GTATACC41200.085.658236
AGTATAC42350.084.903265
TTATACG60650.084.580566
GCTATAG65950.084.122075
CCGTATA43150.083.8795554
CTATAGG66600.083.5854956
TATACTG80750.083.585157
Produced by FastQC (version 0.11.5)