FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3913007
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG71300.090.536155
CGTATAC35350.090.2967155
GTATAGG74450.089.5765846
TATACGG45200.089.114747
GTATACG39500.088.746526
CGTATAG45300.088.498585
CTATACG31500.087.912396
GTATACC34000.087.8746956
GCTATAC41400.086.96835
GGTATAC44100.086.813755
CCTATAC27400.085.8117455
TATAGGC51700.085.6283347
CCTATAG30350.085.452745
TATAGCG51200.085.444047
GTATAGC43150.085.4226466
AGTATAC36600.084.8767555
CTATAGG50050.084.560156
TATAGCC31750.084.228067
ATACGGC8750.084.141498
GCTATAG55000.084.0315255
Produced by FastQC (version 0.11.5)