FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light070.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light070.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4048633
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG76700.090.0437245
GTATAGG79400.089.43456
TATACGG45750.089.3915567
GTATAGC45600.087.91486
CGTATAG46750.087.784235
GCTATAC46150.087.690455
CGTATAC34200.087.359265
CTATACG36150.087.1143346
GTATACG37000.086.910326
CCTATAG30900.086.850915
GTATACC33500.085.923556
TATAGCG50400.085.385097
CCTATAC31900.085.1706165
TATAGGC51500.085.1292657
AGTATAC35600.084.857755
GGTATAC45150.084.793335
ATACGGC9650.084.661428
GCGCTAT14400.084.442653
TATAGCC33000.084.202767
TATAACG37350.083.806797
Produced by FastQC (version 0.11.5)