FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light069.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light069.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000190
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG71900.089.6478655
GTATAGG79300.088.649766
CGTATAG47350.088.177515
CTATACG33850.087.842496
GCTATAC40850.087.789765
CGTATAC32850.087.480045
CCTATAG30400.087.342825
GTATAGC45450.087.26536
TATACGG44750.087.1443257
GTATACG39000.086.351646
GTATACC33100.086.24536
TATAGCG53550.085.685367
GGTATAC44600.085.6268845
TATAGGC50350.084.621747
TATAGCC31950.084.146217
CCGTATA38000.083.999114
TATACTG64250.083.8356867
GTTATAG84400.083.518075
AGTATAC36150.083.436185
CTATAGG50650.083.3702856
Produced by FastQC (version 0.11.5)