FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light068.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light068.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6046640
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG114900.090.491855
GTATAGG121100.089.702756
CGTATAC56800.089.394985
GTATACG63700.089.032526
GTATAGC72600.088.455296
GTATACC59600.088.303366
GCTATAC66750.088.237695
CTATACG58400.088.16586
CGTATAG74700.087.81265
TATACGG78450.087.732237
CCTATAG50200.087.144445
TATAGGC84650.087.0299157
GGTATAC72750.086.96715
TATAGCG93850.086.7987757
TTATACG78000.086.5332956
AGTATAC55800.086.400365
TATACGC56300.086.139257
GCTATAG85850.085.3154755
GATATAG102750.085.1051255
CGTATAA80250.084.75825
Produced by FastQC (version 0.11.5)