FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4493214
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG90350.090.424936
GGTATAG86900.090.407325
CGTATAG53650.089.5980765
CGTATAC39000.089.396255
GTATACG43000.088.591856
TATACGG50950.088.37967
GCTATAC48650.087.87145
CTATACG37900.087.7297066
GTATAGC46400.087.525796
CCTATAG36100.086.709385
GTATACC40350.086.523026
GGTATAC53400.085.570045
TATAGGC59400.084.923117
TATAGCC38550.084.77197
CCTATAC37700.084.541325
AGTATAC41000.084.340355
TATAGCG61000.083.941517
TATACCG55400.083.595467
ATACGGC11200.083.5480048
TTATACG59100.083.505666
Produced by FastQC (version 0.11.5)