FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light066.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light066.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379177
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG69550.090.421055
GTATAGG69700.089.1361166
CTATACG29500.088.878316
TATACGG40200.088.2618947
CCTATAG26300.087.953265
CGTATAC29750.087.652465
CGTATAG41800.087.269745
TATAGCG41050.087.244277
GTATACC30900.087.003496
GTATACG32900.086.47886
GCTATAC35900.086.396765
TATAGGC45050.085.718487
GTATAGC39600.085.641016
GGTATAC39750.085.4373255
GCGCCTA2950.085.411892
AGTATAC32250.083.6560445
CTATAGG43200.083.561956
GCGTATA48300.083.1971364
CCTATAC27400.083.035485
TATAGTG77950.082.931737
Produced by FastQC (version 0.11.5)