FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4572550
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC36100.089.735875
GGTATAG82100.089.271265
GTATACG41100.089.104876
CTATACG38050.088.757256
GTATAGG83500.088.457126
TATACGG50900.088.279987
CCTATAG36250.087.399055
GTATAGC47750.087.040956
CGTATAG49600.086.1885765
GCTATAC45900.085.064445
TATAGCC37300.084.6840747
CCTATAC35350.084.518185
GGTATAC52900.084.4934165
GTATACC37550.084.120266
TTATACG58300.083.674056
TATAGCG52650.083.541367
TATAACG41050.083.196427
GCGTATA61850.083.01854
CTATAGG55450.083.006326
TATAGGC57450.082.845057
Produced by FastQC (version 0.11.5)