FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light064.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light064.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6417600
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG105300.089.313955
CGTATAC47350.089.0790255
GTATAGG104700.088.963816
CCTATAC43300.088.854545
CTATACG47450.088.691096
CGTATAG64500.088.0752645
GTATACG54750.088.0571066
TATACGG65750.087.91797
GCTATAC62550.087.707595
CCTATAG49550.087.520465
GTATAGC64900.086.141866
GTATACC47300.085.357226
GGTATAC66450.085.204745
GCTATAG74150.084.812395
TATAGCG78000.084.401697
GTTATAG134800.083.580945
TTATACG73200.083.5770956
AGTATAC53300.083.501625
TATAGGC72200.083.3531347
TATAGCC50500.083.240447
Produced by FastQC (version 0.11.5)