FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light063.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light063.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3349174
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG65000.091.19985
CTATACG28250.089.955556
CGTATAC28200.089.6097265
GTATAGG69750.089.415586
GCTATAC36200.088.17665
TATACGG37550.087.789437
GTATAGC39050.087.701476
GTATACG28950.087.616396
CGTATAG42100.086.989125
CCTATAG27200.086.966725
GTATACC29750.086.537636
GGTATAC37850.086.340635
TATAGCG43450.085.707527
TATAGGC41750.085.670467
GATATAC38000.084.624825
TATAACG33700.084.14677
CTATAGG42800.084.012666
TATAGCC30600.083.978577
TATACTG57250.083.8819057
GCTATAG47900.083.794185
Produced by FastQC (version 0.11.5)