FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light062.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light062.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867295
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT43510.1125075795872826No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG42040.108706473129151No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG37250.090.789036
GGTATAG70150.090.392685
CGTATAC35850.090.0947345
CTATACG31500.089.568556
GTATACC38500.088.828326
GCTATAC40000.088.822155
GTATAGG82400.088.771426
CCTATAG30350.087.9543465
TATACGG44600.087.755037
GGTATAC43150.087.731835
GTATAGC43450.086.7981346
TATAGGC54800.086.7627267
GCTATAG53150.086.685835
CGTATAG49600.086.6655655
TTATACG49000.085.497256
TATAACG43450.085.158377
AGTATAC36150.085.1424945
GTTATAG87200.084.919875
TATAGCG63250.084.858937
CTATAGG54600.084.7317056
Produced by FastQC (version 0.11.5)