FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5138530
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG97750.091.354085
GTATAGG101700.090.001056
GCTATAC54600.089.952815
CGTATAC44250.089.7387165
CGTATAG62300.088.441645
TATACGG64050.088.324167
CTATACG44350.088.144066
GTATACG52700.087.607746
GTATAGC59100.087.363276
GTATACC50300.087.349536
CCTATAG39400.087.162355
TATAGGC66750.086.7439657
CTATAGG67100.086.008356
GCTATAG70500.085.768175
TATAGCG67500.085.709787
GTTATAG113850.085.360995
CCGTATA48500.085.3027954
AGTATAC47350.085.2679445
TTATACG62100.085.2080156
AGTATAG79050.085.023985
Produced by FastQC (version 0.11.5)