FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light060.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light060.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7055789
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG133800.090.241975
CGTATAG83050.089.908775
GTATAGG145500.089.77576
GCTATAC75150.089.5000845
CGTATAC62400.089.0615845
CTATACG56250.088.243546
TATACGG84100.087.995527
GTATAGC77900.086.7673956
TATAGCG89050.086.731287
GTATACC60800.086.405366
GCTATAG95100.086.208375
TTATACG87550.086.1555946
TATAGGC91000.086.125497
CCTATAG56850.085.724725
GTATACG71400.085.286246
CTATAGG88750.085.04426
GTTATAG150350.084.605055
AGTATAG105450.084.1883245
CCTATAC53950.083.817485
AGTATAC65550.083.622335
Produced by FastQC (version 0.11.5)