FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6751817
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG131150.090.796975
GCTATAC73000.089.727745
CGTATAG74300.089.564245
TATACGG78300.089.235217
GTATAGG132800.088.9892656
CGTATAC53050.088.193415
CTATACG54050.087.352636
CCTATAC50550.087.105165
CTATAGG78950.086.996446
TATAGCG71600.086.573077
GTATAGC73250.086.308936
GTATACG69850.086.293986
GTATACC52150.085.707866
GCTATAG93400.085.640775
TATAGGC80900.085.604057
CCTATAG47400.085.378115
GCGCTAT23200.084.966063
CGCTATA71450.084.760454
TTATACG78600.084.603976
TATAGGT155300.083.46757
Produced by FastQC (version 0.11.5)