FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light058.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light058.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6367697
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG124150.090.522345
CGTATAG71500.089.086215
GTATAGG131250.088.8826146
GCTATAC61350.088.882265
TATACGG75400.088.069117
CTATACG51700.087.189356
CGTATAC53950.086.986785
TATAGCG79200.086.9022457
GTATAGC70650.086.124116
TATAGGC81600.086.092597
CCTATAC45700.085.956175
GTATACG65700.085.383176
CCTATAG45800.085.146235
GCTATAG90550.085.031995
CTATAGG79800.084.939336
TTATACG78050.084.652926
CCGTATA55950.084.216924
GTATACC54050.083.925796
TATACGC51900.083.650127
TATAACG62650.083.550357
Produced by FastQC (version 0.11.5)