FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light057.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light057.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6097795
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG114800.091.606085
GTATAGG118700.090.036876
CGTATAG65250.089.0296255
GCTATAC59850.088.57045
CGTATAC49600.088.295355
TATACGG64700.087.951077
CTATACG47050.087.7301256
TATAGCG66950.087.691297
CCTATAG42850.086.906825
GTATACG58350.086.451456
GCTATAG76500.086.24415
TATAGGC72600.085.7083747
TTATACG70200.085.458416
GTATAGC64450.085.0494466
CTATAGG76200.084.962946
CCTATAC42450.084.928355
GTATACC49100.084.841186
GCGCTAT17100.084.720553
CCGTATA50600.084.672244
GTTATAG136600.084.184615
Produced by FastQC (version 0.11.5)