FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7006487
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG154100.089.911696
GGTATAG144300.089.599215
CGTATAG68500.087.647375
CGTATAC40350.086.639655
TATACGG66550.086.004547
GCTATAC50000.085.4977955
TTATACG75950.085.178846
GTATAGC64600.085.144866
GTATACG63200.085.001766
GGTATAC73950.084.849055
GTTATAG174250.084.393815
ATAGCGC11950.084.2658
GTATAGT182400.083.98226
GCTATAG74600.083.4730455
TATAGGC74400.082.803647
TATACTG109400.082.623557
CCGTATA45050.082.556134
CCTATAG38500.082.536835
AGTATAG99150.082.446185
CTATACG45250.082.4011846
Produced by FastQC (version 0.11.5)