FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5368707
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG106200.090.660295
TATACGG64350.089.241257
CGTATAC45050.089.0942845
GTATAGG114000.088.957176
CTATACG45100.088.46896
CGTATAG63800.088.447115
GTATACG53600.087.997916
TATAGCG61150.087.386427
GCTATAC53550.087.281775
TATAGGC70650.086.661997
CCTATAG40500.085.733445
TTATACG67000.085.569786
GTATAGC59250.085.218296
GTATACC44550.084.869816
GTTATAG118600.084.465595
CCTATAC42200.084.418335
ATACGGC14300.084.368748
CCGCGTA3450.084.027442
AGTATAC47800.083.968575
GCTATAG74600.083.7924655
Produced by FastQC (version 0.11.5)