FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7032459
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG137100.090.530975
CGTATAC58700.089.255015
GTATAGG149700.089.1613546
GCTATAC72500.088.972745
TATACGG89700.088.8569957
CTATACG62550.088.7726756
CGTATAG83300.087.92955
GTATACG72650.087.74236
GTATAGC78600.087.4456256
TATAGCG95400.087.381787
CCTATAG55300.087.355665
GTATACC63600.087.008356
TATAGGC93750.086.386377
TTATACG91650.085.98176
GCTATAG94250.085.475115
CGCTATA72550.085.310444
GTTATAG161100.085.299825
CTATAGG96350.085.238436
AGTATAC62600.085.060225
CCTATAC56550.084.5847555
Produced by FastQC (version 0.11.5)