FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light053.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light053.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6883286
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG130000.089.553525
GCTATAC70050.089.437455
CGTATAG80500.089.038145
GTATAGG138000.088.974216
CTATACG53750.087.839656
GTATACG67600.087.409056
GTATAGC73350.087.0973366
TATACGG74350.087.075837
CGTATAC56200.086.968465
TATAGCG79150.086.8361057
GCTATAG90950.085.701545
CCTATAG50200.085.630325
TATAGGC84750.085.357747
TTATACG81300.085.299226
GTATACC58850.084.827956
CCTATAC49350.084.3139955
TATACTG115850.084.0095447
CTATAGG83350.083.99916
TATAACG66700.083.817247
GTTATAG152900.083.751985
Produced by FastQC (version 0.11.5)