FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5875482
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG112800.091.2510455
TATACGG68550.089.178297
GTATAGG119250.088.7850346
CGTATAC47900.088.751485
CTATAGG77150.088.103216
CGTATAG67550.087.896725
GTATACG60850.087.58316
GTATAGC68000.087.384916
GCTATAG81500.087.1890645
GCTATAC59550.087.181925
GTATACC47450.086.9904566
TATAGCG78250.086.98617
CTATACG47950.086.875846
CCTATAC40700.086.595945
TTATACG69300.085.951286
TATAGGC76200.085.773187
CCTATAG44800.085.2444465
GTTATAG136300.084.613745
GCGCTAT18700.084.584473
CGCTATA63000.084.3679664
Produced by FastQC (version 0.11.5)