FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light051.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light051.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7540139
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT88380.11721269329385041No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG141950.090.9158555
CGTATAG91050.089.833555
GTATAGG160700.089.825616
GTATAGC88500.089.094356
CGTATAC62800.088.796085
CTATACG64000.088.4670646
GCTATAC73350.088.4577565
TATAGCG117750.088.26167
GTATACG78450.088.1563956
TATACGG92150.087.8849267
CCTATAG60300.087.7512365
GCTATAG101700.087.5720755
TTATACG98550.087.093736
GTATACC67400.086.9642266
TATAGGC106250.086.727777
CTATAGG101100.085.930616
AGTATAC70750.085.599085
GTTATAG169800.085.515165
TATACGC71250.084.865067
AGTATAG115150.084.850755
Produced by FastQC (version 0.11.5)