FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6981399
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG120000.090.447465
CGTATAC54500.088.462855
GTATAGG134050.088.444166
TATACGG79750.088.388277
CTATACG55450.088.232196
GCTATAC61750.088.0764855
CGTATAG73350.087.4876255
GTATACG65900.087.4312746
CCTATAG47550.087.3076555
GTATAGC70700.087.273256
TATAGCG87700.087.25447
TATAGGC80700.086.170577
GCTATAG84950.086.164965
GTATACC57400.086.1450356
TTATACG86850.084.49876
ATACGGC16700.084.189998
GTTATAG149900.084.0039145
TATACTG121200.083.94767
CTATAGG89650.083.872966
AGTATAC61250.083.754685
Produced by FastQC (version 0.11.5)