FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4183280
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATTTATTGCTACTGCACTCTTCATTCTCGTTCCTACTGCTTTTTTGC50470.12064695645522175No Hit
TGTTTCTATAAATGTTTCTGTGTTGTTCTCTTTCTCTCTAATGTTGTAAA49350.11796963148534163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG83600.091.24825
GTATAGG87300.089.8836756
CGCGCTA1650.089.291892
TATACGG38950.087.9250957
CGTATAG38850.087.906885
CGTATAC25400.087.143945
GTATAGC35550.086.847796
GCTATAC26950.086.361935
GTATACG34250.086.261076
TATACTG64750.085.534997
GTATAGT113650.085.510846
TATAGGC43000.084.835537
GCTATAG42750.083.44285
GGTATAC39450.083.439425
CCTATAG21600.083.343265
AGTATAC24800.083.314895
CGGTATA47550.083.310654
TTATACG45050.083.1892556
GTTATAG106200.083.011545
CCGTATA28750.082.937494
Produced by FastQC (version 0.11.5)