FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983332
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT31530.10568719807249076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG61200.090.96386
GGTATAG58350.090.603865
GCTATAC31450.090.015645
CGTATAC23900.089.434895
CTATACG27200.089.236886
CGTATAG36500.089.1435855
TATACGG37950.088.616357
GTATAGC33850.088.264156
GCTATAG41750.088.173415
TATAGCG41650.088.157027
CCTATAG23950.088.0582055
GATATAG47850.087.852425
GTATACG30850.087.609156
GGTATAC33700.087.1065755
TATAGGC39050.087.093227
TTATACG37550.087.030386
GTATACC26800.086.669546
GTATAAG53950.084.9626856
AGTATAG45200.084.911275
TATACTG47250.084.846327
Produced by FastQC (version 0.11.5)