FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light030.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light030.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2606831
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG47450.090.393975
GTATAGG51450.090.290366
TATACGG30000.089.14057
CGTATAC21400.088.783895
CTATACG20300.088.68116
GCTATAG33900.088.273185
GCTATAC25650.088.146995
CCTATAG20750.088.131385
CGTATAG29650.088.1101465
GTATACG25300.088.05226
GTATACC21600.087.082196
GCGCTAT8550.086.665533
TATAGCG32900.085.758757
GTATAGC28700.085.565086
AGTATAG37750.085.3101355
TATACGC23050.084.901277
CCGTATA23900.084.664164
GCGTATA36050.084.457984
TATAGGC34150.083.593347
TATAACG26100.083.533397
Produced by FastQC (version 0.11.5)