FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632602
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG47900.090.437455
GTATAGG53850.089.265546
CGTATAG30300.089.198725
CGTATAC23200.089.061565
CTATACG24800.088.67856
GTATAGC28450.088.1537256
GTATACG26950.087.243976
GCTATAC25300.087.11375
GGTATAC30500.086.900725
TATACGG33250.086.856227
CCTATAG21200.085.364665
CCTATAC21600.085.3231665
GTATACC21450.085.255516
TATAGCG36450.084.704187
CTATAGG33850.084.334416
TTATACG32850.084.1543666
GCTATAG35450.084.01185
TATACCG28500.083.665787
TATAGGC36300.083.6148157
CGCGATA2050.083.453382
Produced by FastQC (version 0.11.5)