FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light028.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light028.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3084557
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG52500.089.115265
CGTATAC25200.088.775995
GCTATAC28500.088.3295755
CCTATAC19900.087.596795
TATACGG33650.087.5148547
GTATAGG60550.087.465326
CTATACG23500.087.315446
CCGCGTA1200.087.131892
CGTATAG34050.087.044765
GTATAGC33350.086.5930256
GTATACG29300.086.4041756
TATAGCG36400.086.383697
CTATAGG36650.085.535256
AGTATAG45400.084.1153645
GGTATAC34600.084.013775
GTATACC23650.083.949926
GCTATAG37450.083.2007455
GTATAGT64250.082.945896
GATATAG50900.082.864855
CCTATAG20750.082.635055
Produced by FastQC (version 0.11.5)