FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2586550
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG49450.090.772075
GTATAGG53300.089.473256
TATACGG30650.089.264497
CGTATAG28900.088.095495
GTATACG26350.087.787986
CGTATAC21150.087.5872655
GCTATAC25550.087.37635
GTATAGC27950.087.181096
CCTATAG19200.086.8345645
CTATACG20900.086.81686
CTATAGG31850.086.348716
GCTATAG33050.085.656745
GGTATAC31050.085.513945
GATATAG40850.083.9521565
TATAGGC33250.083.855817
CCTATAC17900.083.853425
GTATACC20650.083.7274866
TATACTG47950.083.3094257
GCGCTAT8050.083.197433
CCGCGTA1200.083.160652
Produced by FastQC (version 0.11.5)