FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light026.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light026.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7363539
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG154950.089.9402545
GTATAGG165350.087.644296
CTATACG59800.087.45246
CTATAGG91600.087.168156
CGTATAG83450.086.517285
CGTATAC58650.086.494575
GTATAGC84000.085.668076
TATACGG91900.085.539777
CCTATAG54350.085.47225
CCTATAC53200.085.4448555
TATAGGG165650.085.3923347
TATACCG69200.085.182687
TATAGGC93150.084.7998357
GGTATAA138800.084.731875
GCTATAG105550.083.2978445
TTATAGC102900.082.996526
AGTATAG118000.082.7611855
TATAGCG89300.082.5516747
GCGTATA106300.082.352674
TATAGGT171950.082.208437
Produced by FastQC (version 0.11.5)