FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2861324
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG54100.089.554545
CCTATAG18300.087.7304465
CGTATAC22200.087.295525
GTATAGG59650.086.796226
CTATAGG33700.086.6822056
TATAGGG58800.085.789067
CTATACG21600.085.762136
GTATAGC31800.085.737246
CGTATAG32100.085.3798755
TATACGG32650.085.105467
TATAGCG34150.084.566357
GGTATAA55950.084.301225
GCTATAG39350.084.134255
GGTATAC32100.083.752185
AGTATAG43500.083.5327765
TATACCG28950.083.512977
CCTATAC18350.083.34985
TTATAGC40700.083.327546
GTATAGT67300.083.2821046
TATAGGT65400.083.1596157
Produced by FastQC (version 0.11.5)