FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11375360
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG208550.090.966765
CTATAGG126600.090.158146
GTATAGG211950.089.5523456
GTATAGC125050.089.1105656
CGTATAG129650.088.3669055
CCTATAG79200.088.101135
CGTATAC100650.087.541715
CTATACG92000.087.5118266
TATAGGG204950.086.932867
CCTATAC79500.086.872475
GGTATAC125900.086.735755
GGTATAA212750.086.5364465
GCTATAG150650.086.390565
TATAGCG154700.086.308867
AGTATAG152800.086.169735
TATACCG98850.085.964387
TATACGG137850.085.729157
TATAGGC134050.085.608127
GATATAG173300.085.295455
GTATACG119600.084.989936
Produced by FastQC (version 0.11.5)