FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5909498
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG109150.090.2128755
GTATAGG117500.088.612636
TATAGCG64700.087.878547
TATACGG68850.087.824857
CTATACG43950.087.5425346
TATAGGC69000.086.601317
GCTATAC53550.086.307055
CGTATAG63900.085.9310155
GTATACG58250.085.622196
GTATAGC61450.085.5694356
CGTATAC45000.085.1832055
CCTATAC38600.084.90925
CTATAGG70200.084.8502966
ATACGCG10750.083.951948
GTATAAG105350.083.682836
CCTATAG41450.083.6548845
TATAGGT139900.083.455927
CGCCCTA2850.083.381182
GGTATAC66750.083.1159745
TATACTG103400.083.010037
Produced by FastQC (version 0.11.5)