FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6148070
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG128000.090.1353155
GTATAGG129750.089.505346
CGTATAG65250.089.1001365
GTATACG64300.087.978846
TATACGG78400.087.9686667
GTATAGC65950.086.8580256
GCTATAC62750.086.6700745
CTATAGG79250.086.2466
CGTATAC48250.086.136695
TATAGCG70700.086.128337
CTATACG48400.085.967886
TATAGGC79800.085.592057
GTATACC48350.085.172626
CCTATAG44900.084.735165
GGTATAC71300.084.0712055
GCTATAG91700.084.0672155
GTTATAG147150.084.0216755
AGTATAC55450.083.346765
TATACTG103950.083.2989657
CCTATAC39550.083.226995
Produced by FastQC (version 0.11.5)