FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6388446
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG113500.090.017915
GTATAGG122850.089.2376
TATACGG68750.087.743587
CGTATAG68250.087.342465
GTATAGC66250.086.824566
GCTATAC61350.086.7137155
TATAGCG72600.086.688937
GTATACG61200.086.460576
CGTATAC46000.086.4275055
CCTATAG45200.086.065645
CCTATAC43550.085.509095
CTATACG50850.085.470156
TATACTG112150.085.214427
TATAGGC76800.084.854967
CTATAGG79000.084.4159246
GCTATAG83500.084.0191655
TATAACG57200.083.621477
GGTATAC71300.083.273125
TATAGGT147100.082.8568347
AGTATAC51800.082.802295
Produced by FastQC (version 0.11.5)