FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7011281
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG131600.090.125785
GCTATAC68800.088.646785
GTATAGG145200.088.5212556
CGTATAG82900.088.122795
GTATACG70250.087.8988956
TATACGG85500.087.831877
CGTATAC58600.086.97385
GTATAGC77550.086.055956
TATAGGC92050.086.019757
CCTATAC51300.085.554135
CCTATAG53250.085.453975
CTATAGG95550.085.4538356
GGTATAC83650.085.061355
CTATACG57500.085.002936
TATAGCG93150.084.799987
GTATACC57500.084.755116
TATACTG118650.084.30967
TTATACG90200.084.2558066
TATAACG72350.083.968897
GCTATAG99250.083.8474665
Produced by FastQC (version 0.11.5)