FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6844896
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG76350.089.0255667
GTATACG65650.088.703336
GGTATAG129100.088.669645
GTATAGG135900.088.636786
GCTATAC59700.087.9964145
CGTATAC51950.087.591945
CGTATAG70550.087.120715
CTATACG55050.086.283316
TATAGGC76800.085.597097
CCTATAG47650.085.129315
TATAGCG85500.084.498167
GCTATAG85900.084.491775
TTATACG86400.084.442616
GTATAGC73400.084.3850866
TATACTG117000.084.117837
GTATACC53100.084.08486
CTATAGG83750.083.65496
TATAACG62200.083.54327
GTATAGT149300.083.417476
GGTATAC76850.083.1927265
Produced by FastQC (version 0.11.5)