FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5328013
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG105600.089.6909565
GTATAGG111550.089.2493446
TATACGG59750.088.479087
CGTATAG55400.088.1395345
GCTATAC45450.087.8920445
GTATACG50300.087.53776
GTATAGC55900.086.500636
CGTATAC38550.086.003975
CCTATAG34100.085.526645
TATAGGC61450.084.871887
GGTATAC55300.084.863175
TATAGCG74350.084.584427
TATACTG87950.084.3044367
ATAGCGC10900.083.666978
GTTATAG125450.083.601845
GTATAGT132600.083.3200456
TTATACG66150.083.293586
GCTATAG60200.083.0843665
TTATAGC80350.082.93836
CTATACG39700.082.913666
Produced by FastQC (version 0.11.5)