FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5677504
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG105950.091.905735
GTATAGG113050.090.167266
CTATACG47850.088.844546
GTATACG55950.088.8017046
CGTATAG64300.088.3506855
TATACGG71300.087.804267
GTATAGC63250.087.564436
GCTATAC58250.087.497065
TATAGCG74450.087.470677
CGTATAC49750.087.170075
TATAGGC73200.086.75817
CCTATAC41000.086.54195
CCTATAG41200.085.3147665
GTATACC46850.085.063266
GCGCTAT17450.084.656143
TTATACG73800.084.636776
TATAACG53950.084.43397
AGTATAG81100.084.398135
GGTATAC63150.084.24315
GCTATAG75800.084.220875
Produced by FastQC (version 0.11.5)