FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_N_Light016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_N_Light016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8445865
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG162450.090.1444555
GTATAGG162750.089.7448046
CGTATAG89000.088.166845
TATACGG95300.087.7711267
GTATACG78400.087.485566
CTATACG66200.087.3208166
GTATAGC88650.087.282586
CCTATAG57350.087.212795
TATAGGC102100.086.903327
CGTATAC68600.086.20465
TATAGCG100850.085.955217
GCTATAC79300.085.893685
GTATACC61350.085.475136
CCTATAC58800.085.062145
GGTATAC89500.084.489985
ATAGCGC17600.084.472798
TTATACG102050.084.385926
CTATAGG102700.084.175596
TATACTG142750.083.68497
GTTATAG194100.083.0805745
Produced by FastQC (version 0.11.5)